Rabbit Interferon gamma ,IFN-gamma ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E06925Rb

Alternative Names

interferon, gamma, IFG, IFI,IFN-gamma,interferon gamma

Reactivity

Rabbit

Detection Range

62.5 pg/mL-4000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of dog TNF-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-105
EDTA plasma (n=4)	95	93-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog TNF-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-105
1:2	Average %	93
	Range %	86-99
1:4	Average %	97
	Range %	90-105
1:8	Average %	95
	Range %	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Oryctolagus cuniculus

Sensitivity

15.6 pg/mL

Limit Of Detection

450 nm

UniProt ID	P30123
Antigen	interferon, gamma

Applications

ELISA

Research Area

IFNG

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10507379	CSB-E06925Rb	96T	$595	Add to Cart
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