Rabbit Interferon Gamma - IFN-G ELISA Kit

Mfr.No.

E0006Rb

Sensitivity

14.24ng/L

Detection Range

30-9000ng/L

Reactivity

Rabbit

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Interferon γ (also known as IFN-γ) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IFN G;IFN gamma;IFNG;IFN-gamma;Interferon gamma

Molecular Weight

19516

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0006Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	571.8	33.61	5.9
Intra-Assay	2	18	2057.8	102.3	5
Intra-Assay	3	18	2440.8	88.14	3.6

Background

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Protocol

These standard curves of E0006Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/L	2.179	2.216	2.157
	2.253
2400ng/L	1.39	1.324	1.265
	1.257
1200ng/L	0.677	0.749	0.69
	0.821
600ng/L	0.505	0.496	0.437
	0.487
300ng/L	0.26	0.226	0.167
	0.191
0ng/L	0.058	0.059	0
	0.06

Gene ID	100008602
UniProt ID	P30123

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110998	E0006Rb	48T	$320		Add to Cart
BHE12110998	E0006Rb	96T	$458		Add to Cart

Rabbit Interferon Gamma - IFN-G ELISA Kit

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