Rabbit Interferon alpha,IFN-alpha ELISA Kit

Mfr.No.

CSB-E07329Rb

Sensitivity

1.56 pg/mL

Detection Range

6.25 pg/mL-400 pg/mL

Reactivity

Rabbit

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Interferon alpha,IFN-alpha, IFN-alpha,interferon alpha

Recovery

The recovery of human PTTG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	106	102-110
EDTA plasma (n=4)	89	86-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PTTG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-97
1:2	Average %	107
	Range %	103-112
1:4	Average %	88
	Range %	83-94
1:8	Average %	86
	Range %	81-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Oryctolagus cuniculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	IFNA1

Storage	Store at 2-8°C. Please refer to protocol.

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