Rabbit intercellular adhesion molecule 1,ICAM-1/CD54 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E06974Rb

Alternative Names

intercellular adhesion molecule 1,ICAM-1/CD54

Reactivity

Rabbit

Detection Range

0.625 ng/mL-40 ng/mL

Sample Type

Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human SC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	85-100
EDTA plasma (n=4)	97	90-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:200	Average %	95
	Range %	80-98
1:400	Average %	96
	Range %	88-105
1:800	Average %	97
	Range %	90-110
1:1600	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Oryctolagus cuniculus

Sensitivity

0.156 ng/mL

Limit Of Detection

450 nm

UniProt ID	U3KPG6
Antigen	intercellular adhesion molecule 1,ICAM-1/CD54

Applications

ELISA

Research Area

ICAM-1, CD54

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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