Rabbit Insulin-like growth factor 1,IGF-1 ELISA Kit

Mfr.No.

CSB-E06994Rb

Alternative Names

insulin-like growth factor 1 (somatomedin C), IGF1A, IGFI, OTTHUMP00000195084, insulin-like growth factor 1

Reactivity

Rabbit

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse REG3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	88	84-93

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse REG3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	102
	Range %	98-106
1:2	Average %	98
	Range %	96-104
1:4	Average %	88
	Range %	86-95
1:8	Average %	96
	Range %	91-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.360	2.512	2.436	2.311
100	1.831	1.987	1.909	1.784
50	1.252	1.254	1.253	1.128
25	0.747	0.766	0.757	0.632
12.5	0.473	0.512	0.493	0.368
6.25	0.315	0.321	0.318	0.193
3.12	0.219	0.217	0.218	0.093
0	0.124	0.126	0.125	?

Species

Oryctolagus cuniculus

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q95222
Antigen	insulin-like growth factor 1 (somatomedin C)

Applications

ELISA

Research Area

IGF1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Construction of engineered corpus cavernosum with primary mesenchymal stem cells in vitro Xie X.et al,Sci Rep,Dec 22

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SKU	Supplier No.	Size	Your price
BHE10507375	CSB-E06994Rb	96T	$615	Add to Cart
BHE10507375	CSB-E06994Rb	5x96T	$2952	Add to Cart
BHE10507375	CSB-E06994Rb	10x96T	$5842.50	Add to Cart

Rabbit Insulin-like growth factor 1,IGF-1 ELISA Kit

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