Rabbit Insulin-like growth factor 1,IGF-1 ELISA Kit

Mfr.No.

CSB-E06994Rb

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Rabbit

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

insulin-like growth factor 1 (somatomedin C), IGF1A, IGFI, OTTHUMP00000195084, insulin-like growth factor 1

Recovery

The recovery of mouse REG3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	88	84-93

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse REG3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	102
	Range %	98-106
1:2	Average %	98
	Range %	96-104
1:4	Average %	88
	Range %	86-95
1:8	Average %	96
	Range %	91-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Oryctolagus cuniculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q95222

Applications	ELISA
Research Area	IGF1

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Construction of engineered corpus cavernosum with primary mesenchymal stem cells in vitro Xie X.et al,Sci Rep,Dec 22

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