Matrices listed below were spiked with certain level of IFN-gamma and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-gamma in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-103
94
EDTA plasma(n=5)
89-98
95
heparin plasma(n=5)
86-105
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-gamma and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IFN-gamma ELISA Kit allows for the in vitro quantitative determination of IFN-gamma concentrations in serum, plasma, tissue homogenates and other biological fluids.