Rabbit ET-1(Endothelin1) ELISA Kit

Mfr.No.

ELK2432

Sensitivity

2.74 pg/mL

Detection Range

7.82-500 pg/mL

Reactivity

Rabbit

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1

Recovery

Matrices listed below were spiked with certain level of recombinant ET-1 and the recovery rates were calculated by comparing the measured value to the expected amount of ET-1 in samples.

Matrix	Recovery range	Average
serum(n=5)	78-92%	85%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	78-96%	87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ET-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-97%	87-96%	86-98%	91-103%
EDTA plasma(n=5)	87-90%	87-98%	85-96%	85-95%
Heparin plasma(n=5)	87-98%	85-96%	92-102%	81-93%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
500.00	2.268	2.175
250.00	1.647	1.554
125.00	1.035	0.942
62.50	0.833	0.740
31.25	0.614	0.521
15.63	0.369	0.276
7.82	0.197	0.104
0.00	0.093	0.000

Sample Type

Cell lysates, Serum, Cell culture supeatants, Tissue homogenates, Plasma, Other biological fluids

Limit Of Detection

500 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	P29560

Applications	ELISA
Research Area	Endocrinology;Cardiovascular biology;

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE15207336	ELK2432	48T	$412		Add to Cart
BHE15207336	ELK2432	96T	$588		Add to Cart

Rabbit ET-1(Endothelin1) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Rabbit ET-1(Endothelin1) ELISA Kit

Rate it now..!!