Matrices listed below were spiked with certain level of ET-1 and the recovery rates were calculated by comparing the measured value to the expected amount of ET-1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-102
97
EDTA plasma(n=5)
98-103
100
heparin plasma(n=5)
89-103
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ET-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
ET-1 ELISA Kit allows for the in vitro quantitative determination of ET-1 concentrations in serum, plasma, tissue homogenates and other biological fluids.