Rabbit Estradiol,E2 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E06915Rb

Sensitivity

62.5 pg/mL

Detection Range

62.5 pg/mL-1000 pg/mL

Reactivity

Rabbit

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Estradiol,E2

Recovery

The recovery of human CTSB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	87	83-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CTSB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	93
	Range %	89-97
1:4	Average %	98
	Range %	94-102
1:8	Average %	88
	Range %	86-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Oryctolagus cuniculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	E2

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Use of Estradiol Promotes Graft-Bone Healing in Rabbit Model of Anterior Cruciate Ligament Reconstruction With a Polyethylene Terephthalate Ligament Chai F.et al,/,Jun 16

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