Matrices listed below were spiked with certain level of Emmprin and the recovery rates were calculated by comparing the measured value to the expected amount of Emmprin in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
100
EDTA plasma(n=5)
88-101
95
heparin plasma(n=5)
85-103
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Emmprin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-104%
86-104%
86-104%
EDTA plasma(n=5)
90-99%
86-99%
84-94%
heparin plasma(n=5)
85-99%
80-97%
85-95%
Sensitivity
< 4.688pg/ml
Notes
For Research Use Only
UniProt ID
Q28740
Applications
ELISA
Applications Notes
Emmprin ELISA Kit allows for the in vitro quantitative determination of Emmprin concentrations in serum, plasma, tissue homogenates and other biological fluids.