Matrices listed below were spiked with certain level of DCN and the recovery rates were calculated by comparing the measured value to the expected amount of DCN in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
20-95
57
EDTA plasma(n=5)
27-99
55
heparin plasma(n=5)
35-102
66
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DCN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
13-76%
0-101%
0-46%
EDTA plasma(n=5)
34-65%
8-97%
0-38%
heparin plasma(n=5)
45-95%
37-64%
7-49%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q28888
Applications
ELISA
Applications Notes
DCN ELISA Kit allows for the in vitro quantitative determination of DCN concentrations in serum, plasma, tissue homogenates and other biological fluids.