Rabbit CTXII(Cross Linked C-Telopeptide Of Type II Collagen) ELISA Kit

Mfr.No.

ELK8441

Sensitivity

52.3 pg/mL

Detection Range

156.25-10000 pg/mL

Reactivity

Rabbit

Sample Type

Cell lysates, Serum, Cell culture supernatants, Tissue homogenates, Plasma, Other biological fluids

Assay Duration

Assay Type

Competitive Inhibition

Alternative Names

CTX-II; CTX2

Recovery

Matrices listed below were spiked with certain level of recombinant CTXII and the recovery rates were calculated by comparing the measured value to the expected amount of CTXII in samples.

Matrix	Recovery range	Average
serum(n=5)	77-95%	86%
EDTA plasma(n=5)	92-106%	99%
Heparin plasma(n=5)	88-103%	91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CTXII and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	89-99%	83-99%	85-105%	87-98%
EDTA plasma(n=5)	85-96%	87-101%	89-102%	85-98%
Heparin plasma(n=5)	93-106%	87-96%	89-101%	82-90%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
10000.00	0.175
5000.00	0.386
2500.00	0.689
1250.00	0.878
625.00	1.257
312.50	1.464
156.25	1.796
0.00	2.219

Limit Of Detection

10000 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rabbit CTXII protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit CTXII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit CTXII in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Antigen	CTXII

Applications	ELISA
Research Area	Metabolic pathway;Bone metabolism;Rheumatology;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15200286	ELK8441	48T	$320		Add to Cart
BHE15200286	ELK8441	96T	$458		Add to Cart

Rabbit CTXII(Cross Linked C-Telopeptide Of Type II Collagen) ELISA Kit

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