Matrices listed below were spiked with certain level of CTX-II and the recovery rates were calculated by comparing the measured value to the expected amount of CTX-II in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
94
EDTA plasma(n=5)
91-102
99
heparin plasma(n=5)
90-98
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CTX-II and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-100%
86-104%
90-105%
EDTA plasma(n=5)
83-100%
84-100%
86-99%
heparin plasma(n=5)
84-97%
80-94%
82-99%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
G1T5V9
Applications
ELISA
Applications Notes
CTX-IIELISA Kit allows for the in vitro quantitative determination of CTX-II concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rabbit CTX-II(Cross Linked C-telopeptide of Type II Collagen)ELISA Kit