Matrices listed below were spiked with certain level of CP and the recovery rates were calculated by comparing the measured value to the expected amount of CP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
97
EDTA plasma(n=5)
85-102
93
heparin plasma(n=5)
86-98
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-103%
89-102%
88-104%
EDTA plasma(n=5)
87-100%
86-96%
86-101%
heparin plasma(n=5)
89-96%
86-100%
85-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
CP ELISA Kit allows for the in vitro quantitative determination of CP concentrations in serum, plasma, tissue homogenates and other biological fluids.