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Home / ELISA Kit

Rabbit complement fragment 5a,C5a ELISA Kit

SKU : BHE10507331

This Human UCN2 ELISA Kit was designed for the quantitative measurement of Human UCN2 protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-E13247Rb

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Rabbit

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

complement fragment 5a,C5a

Recovery

The recovery of human UCN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-99
EDTA plasma (n=4)		104		100-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human UCN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		84
		Range %		80-88
1:2		Average %		90
		Range %		85-95
1:4		Average %		97
		Range %		92-110
1:8		Average %		93
		Range %		86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.258	2.302	2.280	2.142
200	1.735	1.696	1.716	1.578
100	1.126	1.101	1.114	0.976
50	0.678	0.637	0.658	0.520
25	0.412	0.434	0.423	0.285
12.5	0.311	0.309	0.310	0.172
6.25	0.243	0.254	0.249	0.111
0	0.137	0.139	0.138

Species

Oryctolagus cuniculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Protocol

Applications	ELISA
Research Area	C5a

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10507331	CSB-E13247Rb	96T	$595		Add to Cart
BHE10507331	CSB-E13247Rb	5x96T	$2856		Add to Cart
BHE10507331	CSB-E13247Rb	10x96T	$5652.50		Add to Cart

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