Rabbit Complement fragment 3a,C3a ELISA Kit

Rabbit Complement fragment 3a,C3a ELISA Kit

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Mfr.No.

CSB-E09283Rb

Alternative Names

Complement fragment 3a,C3a

Reactivity

Rabbit

Detection Range

6.25 ng/mL-400 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse AOC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	97	94-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AOC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	83
	Range %	80-86
1:2	Average %	90
	Range %	85-96
1:4	Average %	96
	Range %	93-99
1:8	Average %	104
	Range %	100-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
80	2.507	2.569	2.538	2.351
40	1.662	1.600	1.631	1.444
20	0.999	0.968	0.984	0.797
10	0.587	0.572	0.580	0.393
5	0.444	0.458	0.451	0.264
2.5	0.354	0.362	0.358	0.171
1.25	0.279	0.298	0.289	0.102
0	0.188	0.186	0.187

Species

Oryctolagus cuniculus

Sensitivity

1.56 ng/mL

Limit Of Detection

450 nm

Antigen	Complement fragment 3a,C3a

Applications

ELISA

Research Area

C3a

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Novel self-assembled pH-responsive biomimetic nanocarriers for drug delivery. Wu M.et al,Mater Sci Eng C Mater Biol Appl.,64:346-53

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BHE10507330	CSB-E09283Rb	10x96T	$8132	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.