Rabbit Collagen Type IV(COL IV) ELISA Kit

Rabbit Collagen Type IV(COL IV) ELISA Kit

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Mfr.No.

CSB-E14232Rb

Sensitivity

3.12 ng/mL

Detection Range

12.5 ng/mL-800 ng/mL

Reactivity

Rabbit

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Collagen Type IV(COL, Collagen Type 4(COL 4),_x000D_ Collagen Type IV(COL IV), Collagen Type IV(COL, Collagen Type 4(COL 4),_x000D_ Collagen Type IV(COL IV)

Recovery

The recovery of rabbit TRAP5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-96
EDTA plasma (n=4)	103	93-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TRAP5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	88-101
1:2	Average %	88
	Range %	81-94
1:4	Average %	97
	Range %	87-101
1:8	Average %	91
	Range %	84-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?U/ml	OD1	OD2	Average	Corrected
5000	1.994	1.912	1.953	1.835
2500	1.776	1.745	1.761	1.643
1250	1.504	1.561	1.533	1.415
625	1.212	1.178	1.195	1.077
312	0.929	0.900	0.915	0.797
156	0.506	0.498	0.502	0.384
78	0.289	0.282	0.286	0.168
0	0.116	0.120	0.118

Species

Oryctolagus cuniculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	Col

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507328	CSB-E14232Rb	96T	$595	Add to Cart
BHE10507328	CSB-E14232Rb	5x96T	$2856	Add to Cart
BHE10507328	CSB-E14232Rb	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.