Matrices listed below were spiked with certain level of CD40L/TNFSF5 and the recovery rates were calculated by comparing the measured value to the expected amount of CD40L/TNFSF5 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-105
98
EDTA plasma(n=5)
85-99
91
heparin plasma(n=5)
88-99
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD40L/TNFSF5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
CD40L/TNFSF5 ELISA Kit allows for the in vitro quantitative determination of CD40L/TNFSF5 concentrations in serum, plasma, tissue homogenates and other biological fluids.