Rabbit Bone Morphogenetic Protein 2

Mfr.No.

E0318Rb

Sensitivity

0.26ng/L

Detection Range

0.5-200ng/L

Reactivity

Rabbit

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

BMP 2;BMP2;BMP-2;Bone morphogenetic protein 2

Molecular Weight

44664

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0318Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	33.7	1.37	4.1
Intra-Assay	2	18	9.1	0.51	5.5
Intra-Assay	3	18	16	0.92	5.7

Background

Induces cartilage and bone formation. Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4.

Protocol

These standard curves of E0318Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/L	2.325	2.178	2.124
	2.03
60ng/L	1.692	1.766	1.712
	1.839
30ng/L	1.166	1.144	1.091
	1.123
15ng/L	0.746	0.736	0.683
	0.727
7.5ng/L	0.359	0.398	0.345
	0.438
0ng/L	0.047	0.053	0
	0.06

Gene ID	100009349
UniProt ID	O46564

Research Area	Cardiovascular; Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111277	E0318Rb	48T	$320		Add to Cart
BHE12111277	E0318Rb	96T	$458		Add to Cart

Rabbit Bone Morphogenetic Protein 2 - BMP2 ELISA Kit

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