Rabbit basic fibroblast growth factor,bFGF ELISA Kit

Rabbit basic fibroblast growth factor,bFGF ELISA Kit

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Mfr.No.

CSB-E06938Rb

Sensitivity

1.95 pg/mL

Detection Range

7.8 pg/mL-500 pg/mL

Reactivity

Rabbit

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

fibroblast growth factor 2 (basic), BFGF, FGFB, HBGF-2, basic fibroblast growth factor bFGF, fibroblast growth factor 2, heparin-binding growth factor 2, prostatropin

Recovery

The recovery of bovine SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-99
EDTA plasma (n=4)	96	89-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	93
	Range %	89-98
1:4	Average %	95
	Range %	91-98
1:8	Average %	92
	Range %	90-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?g/ml	OD1	OD2	Average
0	1.963	1.977	1.970
0.1	1.644	1.654	1.649
0.4	1.239	1.245	1.242
1.6	0.817	0.798	0.808
5	0.372	0.368	0.370
20	0.121	0.123	0.122

Species

Oryctolagus cuniculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P48799

Applications	ELISA
Research Area	FGF2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507305	CSB-E06938Rb	96T	$615	Add to Cart
BHE10507305	CSB-E06938Rb	5x96T	$2952	Add to Cart
BHE10507305	CSB-E06938Rb	10x96T	$5842.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.