Rabbit Apolipoprotein B - APO-B ELISA Kit

Mfr.No.

E0150Rb

Sensitivity

2.43ug/ml

Detection Range

5-1500ug/ml

Reactivity

Rabbit

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Apolipoprotein B (also known as APO-B) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

APO-B;Apolipoprotein B;Fragment

Molecular Weight

15664

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0150Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	186.85	10.61	5.68
Intra-Assay	2	18	472.82	24.47	5.18
Intra-Assay	3	18	658.64	19.9	3.02

Background

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

Protocol

These standard curves of E0150Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ug/ml	1.66	1.684	1.639
	1.707
400ug/ml	0.877	0.911	0.866
	0.945
200ug/ml	0.575	0.555	0.51
	0.536
100ug/ml	0.223	0.192	0.147
	0.162
50ug/ml	0.08	0.108	0.063
	0.136
0ug/ml	0.036	0.045	0
	0.055

Gene ID	100009101
UniProt ID	P17165

Research Area	Cardiovascular; Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111129	E0150Rb	48T	$320		Add to Cart
BHE12111129	E0150Rb	96T	$458		Add to Cart

Rabbit Apolipoprotein B - APO-B ELISA Kit

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