Rabbit Apolipoprotein A1 - APO-A1 ELISA Kit

Mfr.No.

E0149Rb

Sensitivity

1.21ug/ml

Detection Range

2-700ug/ml

Reactivity

Rabbit

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Apoprotein A1 (also known as APO-A1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

APOA 1;APOA1;ApoA-I;Apo-AI;Apolipoprotein A1;Apolipoprotein A-I;ProapoA-I;Proapolipoprotein A-I;Truncated apolipoprotein A-I;

Molecular Weight

30591

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0149Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	205.9	5.55	2.7
Intra-Assay	2	18	102	5.05	4.9
Intra-Assay	3	18	130.3	6.89	5.3

Background

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

Protocol

These standard curves of E0149Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
400ug/ml	2.388	2.264	2.223
	2.139
200ug/ml	1.361	1.314	1.273
	1.268
100ug/ml	0.618	0.69	0.649
	0.761
50ug/ml	0.321	0.322	0.281
	0.323
25ug/ml	0.158	0.153	0.112
	0.147
0ug/ml	0.043	0.041	0
	0.039

Gene ID	100009253
UniProt ID	P09809

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111128	E0149Rb	48T	$320		Add to Cart
BHE12111128	E0149Rb	96T	$458		Add to Cart

Rabbit Apolipoprotein A1 - APO-A1 ELISA Kit

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