Rabbit Aggrecan core protein(ACAN) ELISA kit

Rabbit Aggrecan core protein(ACAN) ELISA kit

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Mfr.No.

CSB-E12831Rb

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Rabbit

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

aggrecan 1, AGC1, AGCAN, CSPG1, CSPGCP, MSK16, SEDK, cartilage-specific proteoglycan core protein, chondroitin sulfate proteoglycan 1, large aggregating proteoglycan

Recovery

The recovery of rat SDC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	94-107
EDTA plasma (n=4)	100	91-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat SDC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	88-102
1:2	Average %	101
	Range %	95-104
1:4	Average %	90
	Range %	83-94
1:8	Average %	94
	Range %	87-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.458	2.422	2.440	2.264
10	2.004	1.976	1.990	1.814
5	1.307	1.345	1.326	1.150
2.5	0.728	0.737	0.733	0.557
1.25	0.432	0.448	0.440	0.264
0.625	0.333	0.342	0.338	0.162
0.312	0.251	0.243	0.247	0.071
0	0.174	0.178	0.176

Species

Oryctolagus cuniculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q28670

Applications	ELISA
Research Area	ACAN

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507289	CSB-E12831Rb	96T	$595	Add to Cart
BHE10507289	CSB-E12831Rb	5x96T	$2856	Add to Cart
BHE10507289	CSB-E12831Rb	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.