Pregnant mare serum gonadotrophin (PMSG)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13099Hs

Alternative Names

N/A

Reactivity

Horse

Detection Range

5 mIU/mL-2000 mIU/mL

Sample Type

Serum

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of mouse Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-100
EDTA plasma (n=4)	99	95-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	99
	Range %	96-101
1:200	Average %	85
	Range %	81-89
1:400	Average %	100
	Range %	97-103
1:800	Average %	83
	Range %	80-86

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Equus caballus

Sensitivity

5 mIU/mL

Limit Of Detection

450 nm

Antigen	Pregnant mare serum gonadotrophin (PMSG)ELISA Kit

Applications

ELISA

Research Area

PMSG

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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