Matrices listed below were spiked with certain level of PREG and the recovery rates were calculated by comparing the measured value to the expected amount of PREG in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-103
97
EDTA plasma(n=5)
85-105
95
heparin plasma(n=5)
86-101
90
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PREG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PREG ELISA Kit allows for the in vitro quantitative determination of PREG concentrations in serum, plasma, tissue homogenates and other biological fluids.