Porcine Vascular Endothelial Growth Factor A

Mfr.No.

E0470Po

Description

This sandwich kit is for the accurate quantitative detection of Vascular Endothelial Growth Factor A (also known as VEGFA) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Vascular endothelial growth factor A;Vascular permeability factor;VEGF A;VEGFA;VEGF-A;VPF

Reactivity

Porcine

Detection Range

10-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

22368

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.19ng/L

Background

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin (By similarity). Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity).

Gene ID	397157
UniProt ID	P49151
Antigen	VEGFA

Research Area

Cancer

Protocol

These standard curves of E0470Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	1.985	2.017	1.976
	2.049
800ng/L	1.553	1.433	1.392
	1.313
400ng/L	0.595	0.581	0.54
	0.567
200ng/L	0.457	0.418	0.377
	0.378
100ng/L	0.212	0.172	0.131
	0.133
0ng/L	0.045	0.041	0
	0.037

Precision

We measured random samples of E0470Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	571.2	29.42	5.2
Intra-Assay	2	18	1134.4	57.64	5.1
Intra-Assay	3	18	298.1	14.59	4.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110854	E0470Po	48T	$320		Add to Cart
BHE12110854	E0470Po	96T	$458		Add to Cart

Porcine Vascular Endothelial Growth Factor A - VEGFA ELISA Kit

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