Matrices listed below were spiked with certain level of TSH and the recovery rates were calculated by comparing the measured value to the expected amount of TSH in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-102
92
EDTA plasma(n=5)
85-104
93
heparin plasma(n=5)
86-101
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TSH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-103%
86-98%
85-105%
EDTA plasma(n=5)
82-101%
82-95%
84-101%
heparin plasma(n=5)
80-98%
80-99%
85-99%
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Notes
For Research Use Only
Applications
ELISA
Applications Notes
TSH ELISA Kit allows for the in vitro quantitative determination of TSH concentrations in serum, plasma, tissue homogenates and other biological fluids.
Storage
2-8 °C for 6 months
Reviews of Porcine TSH (Thyroid Stimulating Hormone) ELISA Kit