Matrices listed below were spiked with certain level of TCC C5b-9 and the recovery rates were calculated by comparing the measured value to the expected amount of TCC C5b-9 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-102
94
EDTA plasma(n=5)
91-98
94
heparin plasma(n=5)
85-102
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TCC C5b-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-100%
86-105%
91-100%
EDTA plasma(n=5)
89-101%
84-99%
87-95%
heparin plasma(n=5)
84-98%
82-88%
80-98%
Sensitivity
< 4.688ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
TCC C5b-9 ELISA Kit allows for the in vitro quantitative determination of TCC C5b-9 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Porcine TCC C5b-9(Terminal Complement Complex C5b-9) ELISA Kit