Porcine Pulmonary Surfactant-associated Protein B

Mfr.No.

E0599Po

Alternative Names

8 kDa protein;Pulmonary surfactant-associated protein B;Pulmonary surfactant-associated proteolipid SPL(Phe;SFTP3;SP-B

Reactivity

Porcine

Detection Range

7-1500ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

8714

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

3.32ng/mL

Background

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

Gene ID	397296
UniProt ID	P15782
Antigen	SP-B

Research Area

Immunology; Microbiology; Signal Transduction; Cell Biology; Cancer

Protocol

These standard curves of E0599Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/mL	2.345	2.191	2.147
	2.037
400ng/mL	1.716	1.712	1.668
	1.708
200ng/mL	1.198	1.229	1.185
	1.26
100ng/mL	0.659	0.663	0.619
	0.667
50ng/mL	0.436	0.419	0.375
	0.402
0ng/mL	0.053	0.044	0
	0.035

Precision

We measured random samples of E0599Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	77.33	3.95	5.11
Intra-Assay	2	18	216.28	12.79	5.91
Intra-Assay	3	18	248.47	11.62	4.68

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110972	E0599Po	48T	$320		Add to Cart
BHE12110972	E0599Po	96T	$458		Add to Cart

Porcine Pulmonary Surfactant-associated Protein B - SP-B ELISA Kit

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