Matrices listed below were spiked with certain level of PTH and the recovery rates were calculated by comparing the measured value to the expected amount of PTH in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
96
EDTA plasma(n=5)
85-100
92
heparin plasma(n=5)
90-105
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PTH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-102%
92-104%
91-105%
EDTA plasma(n=5)
83-101%
82-99%
85-96%
heparin plasma(n=5)
82-96%
80-98%
88-95%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
P01269
Antigen
PTH
Applications
ELISA
Applications Notes
PTH ELISA Kit allows for the in vitro quantitative determination of PTH concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Porcine PTH(PaRathyroid hormone) ELISA Kit