Matrices listed below were spiked with certain level of PCNA and the recovery rates were calculated by comparing the measured value to the expected amount of PCNA in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-104
98
EDTA plasma(n=5)
93-101
97
heparin plasma(n=5)
89-102
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PCNA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-92%
88-101%
86-100%
EDTA plasma(n=5)
90-101%
89-101%
92-100%
heparin plasma(n=5)
91-100%
90-99%
91-97%
Sensitivity
< 0.375ng/ml
Notes
For Research Use Only
UniProt ID
Gene ID:9823 (NCBI)
Applications
ELISA
Applications Notes
PCNA ELISA Kit allows for the in vitro quantitative determination of PCNA concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Porcine PCNA(Proliferating Cell Nuclear Antigen) ELISA Kit