Porcine Pancreatic Alpha-amylase

Mfr.No.

E0474Po

Sensitivity

1.12ng/ml

Detection Range

2-600ng/ml

Reactivity

Porcine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Pancreatic alpha-amylase (also known as AMY2A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

1,4-alpha-D-glucan glucanohydrolase;AMY2A;AMY2B;PA;Pancreatic alpha-amylase

Molecular Weight

57086

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0474Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	170.94	7.11	4.16
Intra-Assay	2	18	51.97	2.84	5.47
Intra-Assay	3	18	74.86	3.09	4.13

Background

Pancreatic alpha-amylase is an enzyme that in humans is encoded by the AMY2A gene. Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas.

Protocol

These standard curves of E0474Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ng/ml	2.044	2.079	2.009
	2.114
160ng/ml	1.561	1.452	1.381
	1.342
80ng/ml	0.9	0.914	0.843
	0.928
40ng/ml	0.425	0.447	0.376
	0.469
20ng/ml	0.229	0.231	0.16
	0.232
0ng/ml	0.069	0.071	0
	0.072

Gene ID	397397
UniProt ID	P00690

Research Area	Signal Transduction; Tags & Cell Markers; Cancer; Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110858	E0474Po	48T	$320		Add to Cart
BHE12110858	E0474Po	96T	$458		Add to Cart

Porcine Pancreatic Alpha-amylase - AMY2A ELISA Kit

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