Porcine Neurofilament Heavy Polypeptide

Mfr.No.

E0359Po

Sensitivity

0.153ng/mL

Detection Range

0.3-90ng/mL

Reactivity

Porcine

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

200 kDa neurofilament protein;Fragment;NEFH;Neurofilament heavy polypeptide;Neurofilament triplet H protein;NF-H

Molecular Weight

16204

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0359Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	47.05	1.34	2.86
Intra-Assay	2	18	24.15	1.12	4.64
Intra-Assay	3	18	15.8	0.69	4.34

Background

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

Protocol

These standard curves of E0359Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/mL	1.787	1.813	1.776
	1.838
24ng/mL	0.922	0.962	0.925
	1.001
12ng/mL	0.583	0.53	0.493
	0.477
6ng/mL	0.287	0.234	0.197
	0.18
3ng/mL	0.092	0.078	0.041
	0.064
0ng/mL	0.036	0.037	0
	0.038

Gene ID	100156492
UniProt ID	P12037

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110755	E0359Po	48T	$320		Add to Cart
BHE12110755	E0359Po	96T	$458		Add to Cart

Porcine Neurofilament Heavy Polypeptide - NEFH ELISA Kit

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