Matrices listed below were spiked with certain level of MLCK/MYLK and the recovery rates were calculated by comparing the measured value to the expected amount of MLCK/MYLK in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-104
98
EDTA plasma(n=5)
88-104
95
heparin plasma(n=5)
88-98
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MLCK/MYLK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-99%
90-101%
88-100%
EDTA plasma(n=5)
82-97%
83-98%
86-99%
heparin plasma(n=5)
84-94%
85-98%
81-98%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
P79280
Applications
ELISA
Applications Notes
MLCK/MYLKELISA Kit allows for the in vitro quantitative determination of MLCK/MYLK concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Porcine MLCK/MYLK(Myosin Light Chain Kinase)ELISA Kit