Porcine Interleukin 8 - IL-8 ELISA Kit

Mfr.No.

E0123Po

Sensitivity

0.54ng/L

Detection Range

1-380ng/L

Reactivity

Porcine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Interleukin 8 (also known as IL-8) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Alveolar macrophage chemotactic factor I;AMCF-I;Chemokine;C-X-C motif chemokine 8;C-X-C motif ligand 8;IL 8;IL8;IL-8;Interleukin-8;

Molecular Weight

11633

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0123Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	33.1	1.85	5.6
Intra-Assay	2	18	88.9	5.1	5.7
Intra-Assay	3	18	123.8	4.47	3.6

Background

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.

Protocol

These standard curves of E0123Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
200ng/L	2.057	2.096	2.057
	2.134
100ng/L	1.195	1.226	1.188
	1.258
50ng/L	0.764	0.769	0.73
	0.774
25ng/L	0.526	0.495	0.456
	0.463
12.5ng/L	0.258	0.237	0.198
	0.215
0ng/L	0.033	0.039	0
	0.044

Gene ID	396880
UniProt ID	P26894

Research Area	Cardiovascular; Immunology; Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110545	E0123Po	48T	$320		Add to Cart
BHE12110545	E0123Po	96T	$458		Add to Cart

Porcine Interleukin 8 - IL-8 ELISA Kit

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