Porcine Interleukin 2 - IL-2 ELISA Kit

Mfr.No.

E0118Po

Sensitivity

2.49ng/L

Detection Range

5-2000ng/L

Reactivity

Porcine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Interleukin 2 (also known as IL-2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IL 2;IL2;IL-2;Interleukin-2;T-cell growth factor;TCGF

Molecular Weight

17401

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0118Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	195.1	8.89	4.6
Intra-Assay	2	18	284.3	13.82	4.9
Intra-Assay	3	18	659	22.9	3.5

Background

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

Protocol

These standard curves of E0118Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.659	2.63	2.573
	2.601
600ng/L	1.557	1.577	1.52
	1.597
300ng/L	0.817	0.792	0.735
	0.768
150ng/L	0.417	0.42	0.363
	0.423
75ng/L	0.223	0.245	0.189
	0.268
0ng/L	0.051	0.057	0
	0.063

Gene ID	396868
UniProt ID	P26891

Research Area	Immunology

Storage	2-8°C

Reference	The effects of ryanodine receptor 1 (RYR1) mutation on plasma cytokines and catecholamines during prolonged restraint in pigs Journal: Vet Immunol Immunopathol. 2013 Dec 15;156(3-4):176-81.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110540	E0118Po	48T	$320		Add to Cart
BHE12110540	E0118Po	96T	$458		Add to Cart

Porcine Interleukin 2 - IL-2 ELISA Kit

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