Porcine Interferon Gamma - IFN-G ELISA Kit

Mfr.No.

E0100Po

Sensitivity

2.52pg/ml

Detection Range

5-1500pg/ml

Reactivity

Porcine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Interferon γ (also known as IFN-γ) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IFN G;IFN gamma;IFNG;IFN-gamma;Interferon gamma

Molecular Weight

19419

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0100Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	856.6	32.65	3.8
Intra-Assay	2	18	254.6	10.69	4.2
Intra-Assay	3	18	520.3	19.48	3.7

Background

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Protocol

These standard curves of E0100Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
960pg/ml	1.826	1.823	1.762
	1.821
480pg/ml	1.009	1.041	0.98
	1.073
240pg/ml	0.576	0.544	0.482
	0.512
120pg/ml	0.261	0.232	0.171
	0.203
60pg/ml	0.136	0.14	0.079
	0.144
0pg/ml	0.062	0.061	0
	0.06

Gene ID	396991
UniProt ID	P17803

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110522	E0100Po	48T	$320		Add to Cart
BHE12110522	E0100Po	96T	$458		Add to Cart

Porcine Interferon Gamma - IFN-G ELISA Kit

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