Porcine Insulin-like Growth Factors Binding Protein 1

Mfr.No.

E0575Po

Sensitivity

3.36ng/mL

Detection Range

7-1500ng/mL

Reactivity

Porcine

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

IBP-1;IGF-binding protein 1;IGFBP-1;Insulin-like growth factor-binding protein 1

Molecular Weight

28331

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E0575Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	356.03	18.37	5.16
Intra-Assay	2	18	568.9	18.08	3.18
Intra-Assay	3	18	99.92	4.95	4.95

Background

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration (By similarity).

Protocol

These standard curves of E0575Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/mL	2.104	2.243	2.202
	2.381
400ng/mL	1.147	1.177	1.137
	1.208
200ng/mL	0.769	0.72	0.679
	0.67
100ng/mL	0.413	0.412	0.371
	0.411
50ng/mL	0.179	0.192	0.152
	0.206
0ng/mL	0.031	0.041	0
	0.05

Gene ID	397270
UniProt ID	Q75ZP3

Research Area	Signal Transduction; Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110948	E0575Po	48T	$320		Add to Cart
BHE12110948	E0575Po	96T	$458		Add to Cart

Porcine Insulin-like Growth Factors Binding Protein 1 - IGFBP-1 ELISA Kit

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