Matrices listed below were spiked with certain level of HO-1 and the recovery rates were calculated by comparing the measured value to the expected amount of HO-1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-103
96
EDTA plasma(n=5)
97-103
100
heparin plasma(n=5)
85-99
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HO-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-100%
85-94%
85-100%
EDTA plasma(n=5)
83-100%
87-97%
82-94%
heparin plasma(n=5)
83-99%
81-99%
80-92%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
P32394
Applications
ELISA
Applications Notes
HO-1 ELISA Kit allows for the in vitro quantitative determination of HO-1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Porcine HO-1(Heme Oxygenase 1) ELISA Kit