Porcine Erythropoietin - EPO ELISA Kit

Mfr.No.

E0429Po

Sensitivity

0.154mIU/mL

Detection Range

0.3-90mIU/mL

Reactivity

Porcine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Erythropoietin (also known as EPO) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

EPO;Erythropoietin

Molecular Weight

21304

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E0429Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	13.7	0.45	3.3
Intra-Assay	2	18	7	0.35	5.1
Intra-Assay	3	18	4.7	0.23	4.8

Background

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.

Protocol

These standard curves of E0429Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48mIU/mL	2.366	2.32	2.273
	2.273
24mIU/mL	1.889	1.849	1.802
	1.809
12mIU/mL	1.148	1.206	1.159
	1.264
6mIU/mL	0.683	0.648	0.602
	0.614
3mIU/mL	0.396	0.378	0.332
	0.361
0mIU/mL	0.059	0.047	0
	0.034

Gene ID	397249
UniProt ID	P49157

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110820	E0429Po	48T	$320		Add to Cart
BHE12110820	E0429Po	96T	$458		Add to Cart

Porcine Erythropoietin - EPO ELISA Kit

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