Porcine Endothelial Nitric Oxide Synthase

Mfr.No.

E0231Po

Alternative Names

cNOS;Constitutive NOS;EC-NOS;Endothelial NOS;eNOS;Nitric oxide Synthase, endothelial;NOS 3;NOS type III;NOS3;NOSIII

Reactivity

Porcine

Detection Range

1-400IU/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

133406

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.55IU/mL

Background

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets (By similarity).

Gene ID	397557
UniProt ID	Q28969
Antigen	NOS3

Research Area

Neuroscience

Protocol

These standard curves of E0231Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240IU/mL	2.289	2.177	2.13
	2.064
120IU/mL	1.183	1.221	1.175
	1.26
60IU/mL	0.856	0.77	0.723
	0.683
30IU/mL	0.483	0.472	0.426
	0.462
15IU/mL	0.241	0.247	0.201
	0.253
0IU/mL	0.04	0.046	0
	0.052

Precision

We measured random samples of E0231Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	35.2	2.52	7.1
Intra-Assay	2	18	88.9	5.34	6
Intra-Assay	3	18	161.4	5.6	3.5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110639	E0231Po	48T	$320		Add to Cart
BHE12110639	E0231Po	96T	$458		Add to Cart

Porcine Endothelial Nitric Oxide Synthase - ENOS ELISA Kit

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