Porcine Cathepsin B - CATH-B ELISA Kit

Mfr.No.

E0312Po

Description

This sandwich kit is for the accurate quantitative detection of Cathepsin B (also known as cath-B) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Cathepsin B;Cathepsin B heavy chain;Cathepsin B light chain;CTSB

Reactivity

Porcine

Detection Range

0.3-90ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

36901

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.147ng/ml

Background

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (By similarity). Cleaves matrix extracellular phosphoglycoprotein MEPE (By similarity). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity). Has also been implicated in tumor invasion and metastasis (By similarity).

Gene ID	100037961
UniProt ID	A1E295
Antigen	CTSB

Research Area

Others

Protocol

These standard curves of E0312Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/ml	2.093	2.169	2.128
	2.245
24ng/ml	1.162	1.169	1.128
	1.176
12ng/ml	0.699	0.775	0.735
	0.852
6ng/ml	0.435	0.468	0.427
	0.501
3ng/ml	0.201	0.213	0.172
	0.225
0ng/ml	0.024	0.041	0
	0.058

Precision

We measured random samples of E0312Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	12.4	0.82	6.7
Intra-Assay	2	18	30.1	0.95	3.2
Intra-Assay	3	18	20.7	1.2	5.8

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110711	E0312Po	48T	$320		Add to Cart
BHE12110711	E0312Po	96T	$458		Add to Cart

Porcine Cathepsin B - CATH-B ELISA Kit

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