Porcine Aromatic-L-amino-acid Decarboxylase

Mfr.No.

E0457Po

Sensitivity

0.01ng/ml

Detection Range

0.05-15ng/ml

Reactivity

Porcine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Aromatic-L-amino-acid decarboxylase (also known as DDC) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

AADC;Aromatic-L-amino-acid decarboxylase;DDC;DOPA decarboxylase

Molecular Weight

53936

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0457Po within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.66	0.13	4.75
Intra-Assay	2	18	7.24	0.25	3.46
Intra-Assay	3	18	2.91	0.13	4.41

Background

Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.

Protocol

These standard curves of E0457Po are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	1.677	1.781	1.735
	1.885
4ng/ml	0.935	0.957	0.912
	0.98
2ng/ml	0.641	0.585	0.54
	0.53
1ng/ml	0.252	0.228	0.182
	0.203
0.5ng/ml	0.079	0.116	0.07
	0.152
0ng/ml	0.058	0.045	0
	0.033

Gene ID	396857
UniProt ID	P80041

Research Area	Neuroscience; Cancer; Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110843	E0457Po	48T	$320		Add to Cart
BHE12110843	E0457Po	96T	$458		Add to Cart

Porcine Aromatic-L-amino-acid Decarboxylase - DDC ELISA Kit

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