Matrices listed below were spiked with certain level of anti-SARS-CoV2(N) and the recovery rates were calculated by comparing the measured value to the expected amount of anti-SARS-CoV2(N) in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
93
EDTA plasma(n=5)
93-104
96
heparin plasma(n=5)
86-104
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of anti-SARS-CoV2(N) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-104%
86-105%
86-105%
EDTA plasma(n=5)
87-100%
94-101%
86-97%
heparin plasma(n=5)
85-99%
89-94%
88-91%
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Notes
For Research Use Only
Applications
ELISA
Applications Notes
anti-SARS-CoV2antibody ELISA Kit allows for the in vitro quantitative determination of anti-SARS-CoV2(N) concentrations in serum, plasma, tissue homogenates and other biological fluids.
Storage
2-8 °C for 6 months
Reviews of Porcine anti-SARS-CoV2 (N) (Delta B.1.617.2) antibody ELISA Kit