Plant Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E16686Pl

Sensitivity

3.12 ug/mL

Detection Range

3.12 ug/mL-800 ug/mL

Reactivity

Mus musculus, Mouse

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Ribulose-1,5-bisphosphate carboxylase/oxygenase(RuBisCO)

Recovery

The recovery of mouse WNT10B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	84-90
EDTA plasma (n=4)	102	98-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT10B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	99
	Range %	95-103
1:4	Average %	88
	Range %	84-93
1:8	Average %	104
	Range %	97-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Plant

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	RuBisCO

Storage	Store at 2-8°C. Please refer to protocol.

Reference	High antioxidant capability interacts with respiration to mediate two Alexandrium species growth exploitation of photoperiods and light intensities WangHui, et al,Harmful Algae,January 4

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