Plant arachidonic Acid (AA) ELISA kit

Mfr.No.

CSB-EQ027590PL

Sensitivity

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Detection Range

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Assay Duration

1-5h

Alternative Names

arachidonic Acid (AA)

Recovery

The recovery of human SERPINA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	93-104
EDTA plasma (n=4)	94	89-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SERPINA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5000	Average %	92
	Range %	87-98
1:10000	Average %	103
	Range %	98-108
1:20000	Average %	92
	Range %	90-98
1:40000	Average %	94
	Range %	92-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Plant

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Applications	ELISA
Research Area	AA

Storage	Store at 2-8°C. Please refer to protocol.

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