Pig Vascular Endothelial cell Growth Factor,VEGF ELISA KIT

Pig Vascular Endothelial cell Growth Factor,VEGF ELISA KIT

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Mfr.No.

CSB-E12053p

Alternative Names

vascular endothelial growth factor A, RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165, vascular permeability factor

Reactivity

Pig

Detection Range

4.69 pg/mL-300 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human PSMB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	88	82-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PSMB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	104
	Range %	99-108
1:2	Average %	86
	Range %	83-89
1:4	Average %	94
	Range %	89-98
1:8	Average %	93
	Range %	88-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.750	2.852	2.801	2.656
800	2.048	2.147	2.098	1.953
400	1.285	1.383	1.334	1.189
200	0.763	0.785	0.774	0.629
100	0.416	0.424	0.420	0.275
50	0.251	0.262	0.257	0.112
25	0.204	0.210	0.207	0.062
0	0.144	0.145	0.145

Species

Sus scrofa

Sensitivity

1.17 pg/mL

Limit Of Detection

450 nm

UniProt ID	P49151
Antigen	Vascular Endothelial Growth Factor A

Applications

ELISA

Research Area

VEGFA

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507269	CSB-E12053p	96T	$615	Add to Cart
BHE10507269	CSB-E12053p	5x96T	$2952	Add to Cart
BHE10507269	CSB-E12053p	10x96T	$5842.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.