Pig Trichinella antibody(IgG) ELISA Kit

Mfr.No.

CSB-E15020p

Alternative Names

Trichinella antibody(IgG)

Reactivity

Pig

Detection Range

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Sample Type

Serum

Assay Type

Indirect

Assay Duration

1-5h

Recovery

The recovery of human C8A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	104	100-109

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human C8A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	85
	Range %	80-89
1:2000	Average %	94
	Range %	90-98
1:4000	Average %	96
	Range %	92-100
1:8000	Average %	89
	Range %	84-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.563	2.472	2.518	2.373
50	2.272	2.169	2.221	2.076
25	1.729	1.684	1.707	1.562
12.5	1.253	1.293	1.273	1.128
6.25	0.785	0.801	0.793	0.648
3.12	0.464	0.462	0.463	0.318
1.56	0.316	0.320	0.318	0.173
0	0.141	0.148	0.145	?

Species

Sus scrofa

Sensitivity

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Limit Of Detection

450 nm

Antigen	Trichinella antibody(IgG)

Applications

ELISA

Research Area

IgG

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Studies on Prevalence of Trichinellosis in Pigs, Rodents and Humans Panchal, S. G..et al,MAFSU, Nagpur,/

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SKU	Supplier No.	Size	Your price
BHE10507260	CSB-E15020p	96T	$790	Add to Cart
BHE10507260	CSB-E15020p	5x96T	$3792	Add to Cart
BHE10507260	CSB-E15020p	10x96T	$7505	Add to Cart

Pig Trichinella antibody(IgG) ELISA Kit

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