Pig terminal complement complex C5b-9 (TCC C5b-9)ELISA Kit

Mfr.No.

CSB-E15837p

Sensitivity

4.69 ng/mL

Detection Range

18.75 ng/mL-1200 ng/mL

Reactivity

Pig

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

terminal complement complex C5b-9 (TCC C5b-9)

Recovery

The recovery of human IAPP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	102	98-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IAPP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	83
	Range %	80-86
1:2	Average %	102
	Range %	98-106
1:4	Average %	93
	Range %	90-96
1:8	Average %	105
	Range %	101-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Sus scrofa

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	TCC C5b-9

Storage	Store at 2-8°C. Please refer to protocol.

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