Pig serum amyloid A,SAA ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13309p

Reactivity

Pig

Detection Range

19.53 pg/mL-5000 pg/mL

Sample Type

Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human CLDN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-109
EDTA plasma (n=4)	96	90-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CLDN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	90
	Range %	85-95
1:4	Average %	97
	Range %	92-103
1:8	Average %	93
	Range %	88-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Sus scrofa

Sensitivity

19.53 pg/mL

Limit Of Detection

450 nm

UniProt ID	P0DSO0
Antigen	serum amyloid A,SAA

Applications

ELISA

Research Area

SAA

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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